Table 3 Kinetic and stoichiometric parameters of batch cultures for VP6 producers
From: Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae
Parameter | Strain | |||
|---|---|---|---|---|
| Â | PJ.T-6 | W.T-6 | CEN.U-6 | PD.U-6 |
μmax (h-1) | 0.259 ± 0.005 | 0.276 ± 0.019 | 0.251 ± 0.035 | 0.299 ± 0.019 |
VP6 (μg/L) | 214 ± 21 | 154 ± 23 | 80 ± 1 | 181 ± 37 |
Yx/s (g DCW/g glucose) | 0.107 ± 0.002 | 0.105 ± 0.002 | 0.088 ± 0.003 | 0.160 ± 0.001 |
Yp/x (μg VP6/g DCW) | 99.42 ± 7.95 | 73.35 ± 9.61 | 45.47 ± 2.76 | 56.13 ± 11.46 |
Yp/s (μg VP6/g glucose) | 10.68 ± 1.05 | 7.74 ± 1.16 | 4.02 ± 0.40 | 9.03 ± 1.88 |
Specific productivity (μg VP6/gDCW· h) | 4.52 ± 0.36 | 2.82 ± 0.37 | 2.84 ± 0.17 | 2.14 ± 0.44 |
Volumetric productivity (μg VP6/L·h) | 9.70 ± 0.95 | 5.96 ± 0.89 | 5.02 ± 0.50 | 6.88 ± 1.43 |