Figure 6
From: Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae
Analysis of rotavirus-like particles obtained from cesium chloride gradients. A) and B) SDS-PAGE and Western blot analysis of two opalescent bands isolated from yeast extracts of two different cultures (lanes 1 and 2). Yeast extract of the PD.U-C strain as negative control (lane 3). Each lane was loaded with 5 μg of total protein. C) and D) Transmission electron micrographs of rotavirus-like particles recovered by cesium chloride gradients. Samples were stained with 3% uranyl acetate. Magnification 150,000× and 250,000×, respectively.