From: Strain engineering for improved expression of recombinant proteins in bacteria
Method | Strain | Target protein | Reference |
---|---|---|---|
Spontaneous chromosomal mutagenesis | C(1(DE3), C43(DE3) | Bovine OGCP; bovine phosphate carrier; bovine ADP/ATP translocase; Bacillus subtilis PS3 alanine/H+ carrier; E. coli F-ATPase subunits b and c; bovine F-ATPase subunits b, d, α, β, γ, δ, OSCP, F6; bovine F-ATPase inhibitor protein; Aequoria victoria GFP | [58] |
Chromosomal mutagenesis using chemical mutagens or mutator genes | EXP-Rv1337-1, EXP-Rv1337-2, EXP-Rv1337-3, EXP-Rv1337-4, EXP-Rv1337-5 | Mycobacterium tuberculosis Rv1337, M. tuberculosis Rv2746, M. tuberculosis Rv2835, M. tuberculosis Rv0110, Methanocccus jannaschii rhomboid (MJR), Drosophila melanogaster rhomboid 1 (Rho1) | [60] |
 | GS1, TM1, TM2, TM3, TM4, TM5, TM6 | Variants of human IgG1 | [61] |
Transposon mutagenesis | GS101 (MC4100A dnaJ 349::Tn5 (KanR)) GS102 (MC4100A dinG 1377::Tn5 (KanR)) GS103 (MC4100A nhaR 63::Tn5 (KanR)) GS104 (MC4100A ΔdinG) GS105 (MC4100A ΔdinG dnaJ 349::Tn5 (KanR)) | Human central cannabinoid receptor (CB1) | [62] |
Co-expression of the ASKA library | MC4100A (+ybaB) MC4100A (+yciQ) MC4100A (+glpQ)* | Human bradykinin receptor 2 (BR2), CB1, human neurokinin(substance P) receptor 1 (NKR1), E. coli YidC, E. coli CstA, human stearoyl-CoA desaturase (SCD) | [64] |