Figure 1From: Strain engineering for improved expression of recombinant proteins in bacteriaPeriplasmic expression with cytometric sorting (PECS) for enhanced recombinant protein expression. E. coli cells expressing the protein of interest in the periplasm are incubated in a high-osmolarity buffer that renders their outer membrane permeable to a fluorescently labeled ligand. Cell fluorescence is proportional to the number of functional, ligand-binding molecules in the periplasm. Clones containing genetic lesions that increase protein expression, display higher fluorescence and can be rapidly isolated using FACS. Adapted from Makino et al. [61].Back to article page