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Figure 1 | Microbial Cell Factories

Figure 1

From: Strain engineering for improved expression of recombinant proteins in bacteria

Figure 1

Periplasmic expression with cytometric sorting (PECS) for enhanced recombinant protein expression. E. coli cells expressing the protein of interest in the periplasm are incubated in a high-osmolarity buffer that renders their outer membrane permeable to a fluorescently labeled ligand. Cell fluorescence is proportional to the number of functional, ligand-binding molecules in the periplasm. Clones containing genetic lesions that increase protein expression, display higher fluorescence and can be rapidly isolated using FACS. Adapted from Makino et al. [61].

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