RI protein activities in total soluble and periplasmic fractions [in kU (gCWW)-1] of E. coli RV308 pCUlac-pelB-RI and E. coli RV308 pCUlac-His
-RI after EnBase cultivation in shake flasks with RI induction for 4 h at 22°C without or with 12 mM DTT (A), and SDS-PAGE images of total (T), soluble (S) and insoluble (IN) protein extracts (normalised to equal amounts protein) (B). Left gel: E. coli RV308 dsbA+ pCUlac-pelB-RI (periplasmic expression construct); right gel: E. coli RV308 pCUlac-His6-RI (cytoplasmic production construct). Lanes: 1 - total protein fraction 10 min before induction, 2 to 4 - soluble, total, and insoluble protein fractions 4 h after induction with 12 mM of DTT. Protein size marker: PageRuler™ Protein Ladder Plus (Fermentas). Data originate from three experiments.