Figure 5
RI protein activities in total soluble fractions (A) and SDS-PAGE images of normalised total (T), soluble (S) and insoluble (IN) cell extracts (B) after 4 h of batch cytoplasmic RI production in shake flask batch cultures in E. coli RV308 pCUlac-His 6 -RI at 22°C with 0, 2, 6, 12 or 18 mM of DTT. Lanes in (B): 1 - total protein fraction 10 min before induction, lanes 2 to 4 - soluble, total and insoluble protein fractions 4 h after induction. Gels represent protein fractions after 4 h of RI production without DTT at 37°C (gel 1) or 22°C (gel 2), or with 12 mM DTT at 37°C (gel 3) or 22°C (gel 4).