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Figure 4 | Microbial Cell Factories

Figure 4

From: Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

Figure 4

RI protein activities in total soluble and periplasmic fractions after 4 h of batch RI production in different E. coli RV308 dsbA+ (A) and dsbA- (B) constructs at 22°C with 0, 2, 6, 12 or 18 mM of DTT. Culture conditions: glucose-MSM medium, induction with 0.2 mM IPTG, preinduction temperature 37°C, shift to the respective temperature at the time of induction.

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