Table 2 Pimers used in this study
Name | Sequence (5'-3') * |
|---|---|
RepF | CACAAAACGCCCTACAA |
RepR | GACCCGACAAATGATT |
ORF3F | ACTTACATTGTCATCC |
ORF3R | CCTTCACAGTGTCTTG |
CB | CGCGGATCC ACGCACCACCCCGTCAGTAG (BamHI) |
CE | CCGGAATTC GAAGCACACGGTCACACTG (EcoRI) |
EMF | AACTGCAG CTAATTTTATAAGGAG (PstI) |
EMR | ACGCGTCGAC GTTAAGGGATGCAG (SalI) |
AF | ACGCGTCGAC TTTACCCTTACGCATAC (SalI) |
AR | CGGGATCC TTACGCTTCGCTTGTCTG (BamHI) |
BF | ACGCGTCGAC AAGACACTGTGAAGGC (SalI) |
BR | CGGGATCC TGCCTTTAAGATTTGC (BamHI) |
CR | CGGGATCC AGTCATTCTTGATTAAGTAC (BamHI) |
DR | CGGGATCC TTTCATTATTGCCTCG(BamHI) |
ER | CGGGATCC CTGATACCACCATACTG(BamHI) |
CF | ACGCGTCGAC GAATGGTTAGCCCTTAAG (SalI) |
PucF | CGGAATTC GGAGCTGCATGTGTC (EcoRI) |
PucR | ACGCGTCGAC TCTTCCGCTTCCTC (SalI) |