Table 1 Plasmids used in this study
Plasmids | Relevant characteristics | Source or reference |
|---|---|---|
pGEM-T easy | Cloning vector, Apr | Promaga |
pMD19T simple | Cloning vector, Apr | Takara |
pMG36e | Erythromycinr gene source for vector construction | [17] |
pBT | CAT cassette source for vector construction | Stratagene |
pUC19 | Cloning vector, Apr | Takara |
pSM429 | Cryptic plasmid from P. sp. BSi20429 | This study |
pWD | E. coli-Pseudoalteromonas shuttle vector | This study |
pWD1 | pWD derivative, 1500-bp fragment deleted from pWD | This study |
pWD2 | pWD derivative, 250-bp fragment deleted from pWD1 | This study |
pWD3 | pWD derivative, 150-bp fragment deleted from pWD2 | This study |
pWD4 | pWD derivative, 320-bp fragment deleted from pWD3 | This study |
pWD5 | pWD derivative, 280-bp fragment downstream deleted from pWD4 | This study |
pWD6 | pWD derivative, 140-bp fragment upstream deleted from pWD4 | This study |
pWD-ermC | pWD2-derived ermC expression vector, Plac-ermC fusions | This study |
pWD-cd | pWD2-derived cd expression vector, Plac-cd fusions | This study |