Figure 5
Expression of the recombinant ErmC protein in SM20429 and E. coli with the constructed vector pWD-ermC. (A) SDS-PAGE analysis of the expressed ErmC protein in the total-cell lysates of recombinant SM20429 and E. coli. Lane 1, SM20429 harboring pWD2, used as a negative control; Lane2, SM20429 harboring pWD-ermC; Lane3, E. coli DH5α harboring pWD-ermC, used as a positive control. The SDS-PAGE gel was stained with Coomassie blue. Arrow denotes the band of the expressed ErmC. (B) Analysis of the ErmC-resistance of the transformed SM20429. 1, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 2, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 30 μg/ml EM; 3, pWD-ermC-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml EM; 4, pWD2-harboring SM20429 inoculated in 2216E medium supplemented with 60 μg/ml.