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Table 1 Fermentation characteristics of the wild type E. coli, and its Δcyo A, Δcyd B, Δfn r, and Δfur mutants under aerobic continuous at dilution rate 0.2h-1: C/N ratios (a) 1.68, (b) 8.42. Biomass (g/l) indicates total cells in the fermentor, while cell yield (g/g) reflects biomass formed on the consumed glucose.

From: Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures

 

Wild

ΔcyoA

ΔcydB

Δfnr

Δfur

(A) C- limitation (C/N ratio = 1.68)

Biomass (g/l)

3.33 ± 0.17

2.86 ± 0.14

3.08 ± 0.15

2.38 ± 0.12

2.63 ± 0.13

Glucose (g/l)

ND*

ND*

ND*

ND*

ND*

Acetate (g/l)

1.73 ± 0.09

1.64 ± 0.08

1.25 ± 0.06

2.06 ± 0.10

1.81 ± 0.09

Cell yield (g/g)

0.330 ± 0.02

0.286 ± 0.01

0.308 ± 0.02

0.238 ± 0.01

0.263 ± 0.01

Specific glucose consumption rate (mmol/gdcw.h)

3.33 ± 0.17

3.88 ± 0.19

3.60 ± 0.18

4.66 ± 0.24

4.21 ± 0.21

Specific acetate production rate (mmol/gdcw.h)

1.73 ± 0.09

1.91 ± 0.10

1.35 ± 0.07

2.88 ± 0.14

2.29 ± 0.11

Specific CO2 production rate (mmol/gdcw.h)

6.74 ± 0.34

7.45 ± 0.37

7.99 ± 0.40

9.00 ± 0.45

8.05 ± 0.40

(B) N- limitation (C/N ratio = 8.42)

Biomass (g/l)

1.67 ± 0.08

1.56 ± 0.08

1.62 ± 0.08

1.48 ± 0.07

1.40 ± 0.07

Glucose (g/l)

3.03 ± 0.15

3.01 ± 0.15

3.22 ± 0.16

3.59 ± 0.18

3.68 ± 0.18

Acetate (g/l)

1.41 ± 0.07

1.58 ± 0.08

1.13 ± 0.06

1.32 ± 0.07

1.62 ± 0.08

Cell yield (g/g)

0.240 ± 0.01

0.223 ± 0.01

0.239 ± 0.01

0.231 ± 0.01

0.221 ± 0.01

Specific glucose consumption rate (mmol/gdcw.h)

4.65 ± 0.23

4.98 ± 0.25

4.67 ± 0.13

4.80 ± 0.24

5.02 ± 0.25

Specific acetate production rate (mmol/gdcw.h)

2.82 ± 0.14

3.38 ± 0.17

2.33 ± 0.12

2.98 ± 0.15

3.86 ± 0.19

Specific CO2 production rate (mmol/gdcw.h)

8.25 ± 0.41

8.88 ± 0.44

8.47 ± 0.42

9.23 ± 0.46

8.39 ± 0.42

  1. ND* = Not detectable