Table 2 Primer and hydrolysis probe sequences for qPCR, amplicon length and PCR efficiencies
Gene | Primer 1 (5' - 3') | Primer 2 (5' - 3') | Amplicon length | Hydrolysis probe | Efficiency cDNA | Efficiency plasmid |
|---|---|---|---|---|---|---|
crtW148 | ttgtgcccaaacaattagcc | ttcctcgtggtagccaaaat | 73 | #119 | 1.88 | 1.83 |
crtZ | cgatctttatgccgtggttt | ggccacattcctgtactgc | 72 | #18 | 1.95 | 1.91 |
hpd | ctatctgatcgaccgcttcg | ccttcgatgaagttgaagtcg | 66 | #8 | 1.94 | 2 |