Table 1 Bacterial strains and plasmids

E. coli DH5α

F^-, ϕ80d, lacZ ΔM15, endA 1, recA 1, hsdR 17(r_K^-m_K^-), supE 44, thi-1, gyrA 96, relA 1, Δ(lacZYA-argF)U169

laboratory strain

E. coli BW-CARO

E. coli BW25113, malEG::crtE, fucIK::crtB,

xylAB::crtI, lacZA::crtY

[24]

E. coli BW-CAROdxs

E. coli BW25113, malEG::crtE, fucIK::crtB,

xylAB::crtI, lacZA::crtY, P_T5-dxs

this study

E. coli BW-CAROdxs-idi

E. coli BW25113, malEG::crtE, fucIK::crtB,

xylAB::crtI, lacZYA::crtY, P_T5-dxs, galEM::idi

this study

E. coli BW-CANT

E. coli BW25113, malEG::crtE, fucIK::crtB,

xylAB::crtI, lacZA::crtY, P_T5-dxs, galEM::idi, melAB::crtW148

this study

E. coli BW-ASTA

E. coli BW25113, malEG:: P_tac-crtE, fucIK:: P_tac-crtB,

xylAB:: P_tac-crtI, lacZA:: P_tac-crtY, P_T5-dxs, galEM:: P_tac-idi, melAB:: P_tac-crtW148, rbsDK:: P_rha-crtZ

this study

Plasmids

pKD46

P_araB γ β exo (red recombinase), Amp^R

[29]

pPQE32-148

pPQE32, N. punctiforme PCC 73102 crtW148148 gene, Amp^R

[20]

pJF119ΔN

cloning vector, RBS, P_tac, Amp^R

[26]

pCAS1

pET11a, P. putida KT2440 hpd gene, Amp^R,

[26]

pJF119-idi

pJF119ΔN, E. coli idi gene, Amp^R

this study

pJF119-idi-FRT-cat-FRT

pJF119ΔN, E. coli idi gene, FRT-sites, Amp^R, Cm^R

this study

pQE31

cloning vector, RBS, P_T5, Amp^R

Qiagen, Germany

pCAS30-FRT-cat-FRT

pJF119ΔN, P. ananatis crtE gene, FRT-sites, Amp^R, Cm^R

[27]

pJF119-crtW148

pJF119ΔN, N. punctiforme crtW148 gene, Amp^R,

this study

pJF119-crtW148-FRT-cat-FRT

pJF119ΔN, N. punctiforme crtW148 gene, FRT-sites, Amp^R, Cm^R

this study

pAW-crtZ

pAW223, P. ananatis crtZ gene, Amp^R

this study

pAW-crtZ-FRT-cat-FRT

pAW223, P. ananatis crtZ gene, FRT-sites, Amp^R, Cm^R

this study