Lactococcus lactis M4, a potential host for the expression of heterologous proteins

Table 1 Bacterial strains and plasmids

Strain or plasmid	Characteristic	Source or reference
L. lactis strains
M2, M4, M5, M6, M11, M12, M14, M16	Milk isolates	This study
MG1363	L. lactis subsp. cremoris, plasmid free	[50]
ATCC 11454	L. lactis subsp. lactis	[25]
E. coli strains
TOP10		Invitrogen
Plasmids
pMG36e	Em^r, lactococcal expression vector with constitutive promoter P₃₂	[51]
pMG36e-GFP	Em^r, a derivative of pMG36e carrying GFP gene	Microbial Biotech Laboratory, UPM
pAR1411	Em^r, lactococcal expression vector	[52]
pAJ01	Em^r, naturally occuring lactococcal plasmid isolated from chicken cecum	[53]

ISSN: 1475-2859