Figure 5
From: Production of Nα-acetylated thymosin α1 in Escherichia coli
SDS-PAGE of Tα1 expression and purification. Lane 1: molecular weight marker; Lane 2: non-induced bacterial lysate cultured in LB medium; Lane 3: induced bacterial lysate cultured in FML medium; Lane 4: Tα1-Intein purified by Ni-Sepharose; Lane 5: Tα1-Intein incubated with 100 mM β-mercaptoethanol (ME) at 42°C for 24 h; Lane 6: combined through fractions, after Phenyl Sepharose 6 Fast Flow column; Lane 7: combined eluted fractions, after SORCE30 RPC column; Lane 8: combined eluted fractions, after Q Sepharose Fast Flow column.