Figure 3
From: Production of Nα-acetylated thymosin α1 in Escherichia coli
Induction of cleavage at the N-terminus of the intein in the Tα1-Intein fusion protein. A. Cleavage of purified Tα1-Intein fusion protein was induced at various temperatures for 24 h. "M", molecular weight marker; "control", pre-incubation sample. B. The purified Tα1-Intein fusion protein was incubated at different temperatures for 24 h. "control", pre-incubation sample; "-", without β-ME or DTT; "ME", with 100 mM β-ME; "DTT", with 100 mM DTT.