Figure 4
Gluconate metabolism in P. putida strains KT2440 and KT2442. A. The route before gluconate depletion is indicated by the numbers "1" and the route after gluconate depletion one with "2". The dotted arrows describe the direct transport of gluconate into the cytoplasm which is possible but of minor importance. This figure was adapted from Daddaoua et al. [31]. Abbreviations: Eda = 2-keto-3-deoxygluconate aldolase, Edd = phosphogluconate dehydratase, Gad = gluconate dehydrogenase, Glc-1P = glucose-1-phosphate, Gln = gluconate, Gln-6P = 6-phosphogluconate, GntP = gluconate permease, Gnuk = gluconokinase, G3P = glyceraldhyde 3-phosphate, KDPG = 2-keto-3-deoxy-6-phosphogluconate, 2-KGln = 2-ketogluconate, 2-KGln-6P = 2-keto-6-phosphogluconate, KguD = 2-ketogluconate reductase, KguK = 2-ketogluconate kinase, KguT = 2-ketogluconate transporter, PYR = pyruvate, TCA = tricarboxylic acid cycle. B. Specific uptake rate of gluconate (qC(Gln)), specific production rate of 2-ketogluconate (qC(2-KGln)), carbon specific uptake rate (qC*) and nitrogen specific uptake rate (qN) for P. putida KT2440 growing exponentially on gluconate in bioreactor. The width of the arrows expresses the actual values. C. Same as B but for P. putida KT2442.