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Table 1 Comparison of the specific binding activity in other GPCRs expressed from P. pastoris and Sf9 insect cells system

From: Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

GPCR

P. pastoris

Sf9

Ligand

hADORA2A

220.81 ± 17.05

239.23 ± 47.12

ZM241385a)

hTACR2

61.96 ± 9.83

9.41 ± 2.46

SR48968a)

hDRD2

7.75 ± 1.54

5.98 ± 1.17

Spiperonea)

hAGTR2

3.69 ± 0.42

1.66 ± 0.08

Angioteninb)

rTACR2

36.60 ± 12.99

14.2 ± 5.41

SR48968a)

  1. All GPCRs were expressed under small-scale culture conditions. Each membrane was prepared from cells, and the specific binding assay was determined using radioactive-labeled specific ligand respectively. hADORA2A, human adenosine A2a receptor; hTACR2 and rTACR2, human and rat tachykinin receptor 2, respectively; hDRD2, human dopamine receptor D2; hAGTR2, human angiotensin receptor type 2. a, antagonist; b, agonist. The data are presented as pmol/mg-membrane and mean ± S.D. (n = 3 each).