GPCR |
P. pastoris
| Sf9 | Ligand |
---|
hADORA2A | 220.81 ± 17.05 | 239.23 ± 47.12 | ZM241385a) |
hTACR2 | 61.96 ± 9.83 | 9.41 ± 2.46 | SR48968a) |
hDRD2 | 7.75 ± 1.54 | 5.98 ± 1.17 | Spiperonea) |
hAGTR2 | 3.69 ± 0.42 | 1.66 ± 0.08 | Angioteninb) |
rTACR2 | 36.60 ± 12.99 | 14.2 ± 5.41 | SR48968a) |
- All GPCRs were expressed under small-scale culture conditions. Each membrane was prepared from cells, and the specific binding assay was determined using radioactive-labeled specific ligand respectively. hADORA2A, human adenosine A2a receptor; hTACR2 and rTACR2, human and rat tachykinin receptor 2, respectively; hDRD2, human dopamine receptor D2; hAGTR2, human angiotensin receptor type 2. a, antagonist; b, agonist. The data are presented as pmol/mg-membrane and mean ± S.D. (n = 3 each).