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Figure 5 | Microbial Cell Factories

Figure 5

From: Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Figure 5

Solubilization and purification of CHRM2 from P. pastoris. In each step of the purification process, CHRM2 was subjected to a specific binding assay (A) and CBB staining after SDS-PAGE (B). The specific binding assay was performed using the membrane fraction (memb.), solubilized fraction (sol.), TALON elution fraction (Co elution), and ConA flow-through fraction (ConA FT). Lanes 1-3 indicate fraction numbers 1-3 of the TALON elution. Lane 4 indicates the ConA flow-through fraction.

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Microbial Cell Factories

ISSN: 1475-2859

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