Figure 2

Comparison of culture conditions in P. pastoris and Sf9 insect cells. In P. pastoris, cell density (A), methanol concentration (B), and pH (C) were measured until 5 days after methanol induction. Cell density was estimated from OD₆₀₀. Methanol concentration was monitored before and after methanol addition using a methanol sensor system (RAVEN Biotech Inc.). In Sf9 insect cells, cell density (D), glucose concentration (E), and pH (F) were measured during the period from preculture (4 days) to baculovirus infection (2 days). Cells were stained with 0.25% trypan blue in PBS (-) and living cells were counted with a hemocytometer.