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Figure 8 | Microbial Cell Factories

Figure 8

From: Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

Figure 8

Bioprocess monitoring: Viability and physiology. Viability investigations of a bioreactor cultivation of B. megaterium cells producing/secreting ABF scFv D1.3 (15 g/L fructose, DO>20%, 1L). A) DiBAC4(3)/PI stain of samples, percentages of PI+ and PI- cells according gate analysis of FL1-FC and FL3-FC dot plots. B) Side Scatter (SS), Electronic Volume (EV) mean value measurements related to cell dry weight characteristics at different growth phases. C) Population analysis of cells at late cultivation time points.

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Microbial Cell Factories

ISSN: 1475-2859

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