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Figure 4 | Microbial Cell Factories

Figure 4

From: Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

Figure 4

Production intensity assay-validation. Lysozyme treatment of B. megaterium cells producing antibody fragment D1.3 scFv for assay evaluation distinguishing between producing and non-producing cells. A), B) different lysozyme concentrations and their effect on staining intensity with second antibody (AB) Alexa Fluor 488. C) Immuno-FESEM picture of ABF D1.3 scFv secreting, lysozyme treated B. megaterium cells incubated with a second gold coupled detection antibody. D) Confocal Laser Scanning Microscopy (CLSM) picture of B. megaterium cells, lysozyme treated combined with Alexa Fluor 488 as second detection antibody.

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Microbial Cell Factories

ISSN: 1475-2859

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