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Figure 3 | Microbial Cell Factories

Figure 3

From: Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

Figure 3

Viability estimation. Live/dead test of B. megaterium cells producing ABF D1.3 scFv with different dye combinations of DiBAC4(3)/PI and Syto9/PI. A) DiBAC4(3)/PI stain: 50% dead cell, 50% live cell mixture, B) Syto9/PI stain: 50% dead cell, 50% live cell mixture. C), D) Calibration curves were determined via different mixtures of dead (heat killed) and live (exponential phase) cells.

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Microbial Cell Factories

ISSN: 1475-2859

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