Figure 5
From: Surface display of Salmonella epitopes in Escherichia coli and Staphylococcus carnosus
Presence of the His 6 tag in fusion proteins located in the outer membrane fraction of E. coli. Western blot showing: Lane 1: Positive control; Bacillus Lipase 19.3 kDa [37]; Lane 2: H:gm; Lane 3: SefA; and Lane 4: Negative control: AIDAC. All proteins carry the His6 tag. The molecular sizes of marker proteins are indicated to the left (kDa). Western blot detection with Abcam 6× His tag specific antibody and HisProbe™-HRP.