Schematic representation of the vectors used in this work. Above: structure of the E. coli vector with the anchoring domain AIDAC, a linker region (L), a His6 tag (H) and the specific signal sequence for the AIDA system (SP). Below: structure of the staphylococcal surface display vector with the anchoring regions (X, M), the albumin-binding domain (ABD), a propeptide and the signal sequence from S. hyicus (SP). The passenger is either SefA or H:gm in either vector. The N-terminal is facing to the left and the anchoring C-terminal to the right. The relative sizes of the vector parts are approximate.