Table 3 Oligonucleotides used for cloning
Primer name | Primer sequence (5'>3') | Tm °C | Restriction enzyme site |
|---|---|---|---|
ackaF | GGCCCGGG ATGTCGAGTAAGTTAG | 58.0 | SmaI |
ackaR | TGGCAAGCTT ACATTCAGGCAGTCAGGCGGCTCG | 60.0 | HindIII |
gpmaF | CCGGGTACC ATGGCTGTAACTAAGCTGGTTCTG | 66.9 | KpnI |
gpmaR | CGCGGTCGAC TTACTTCGCTTTACCCTGG | 65.7 | SalI |
fbabF | TCCGGTACC ATGACAGATATTGCGCAGTTGCTTG | 65.6 | KpnI |
fbabR | GGCCGTCGAC TCAGGCGATAGTAATTTTGC | 64.4 | SalI |
pgiF | GCCCGGG ATGAAAAACATCAATCCAACGCAGACC | 66.8 | SmaI |
pgiR | CGGAAGCTT TGATTAACCGCGCCACGCTTTATAG | 65.6 | HindIII |
ptaF | CGGAGGAGGTACC ATGTCCCGTATTATTATG | 63.0 | KpnI |
ptaR | GACGAAGCTT AGATTACTGCTGCTGTGCAGAC | 64.4 | HindIII |
tktbF | CGGAGGGTACC ATGTCCCGAAAAGACCTTG | 54.0 | KpnI |
tktbR | GCGCAAGCTT TCAGGCACCTTTCACTCCC | 57.0 | HindIII |
acebF | GAGCGGTACC ATGACTGAACAGGCAACAACAAC | 58.0 | KpnI |
acebR | TGTGTCGAC TTACGCTAACAGGCGGTAGCCTGG | 58.0 | SalI |