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Table 2 Plasmids employed and constructed in this study

From: Genetic response to metabolic fluctuations: correlation between central carbon metabolism and DNA replication in Escherichia coli

Plasmid Relevant characteristics Reference
pBAD24 Ori pBR322; bla+ PBAD [29]
pAS101 pBAD24 bearing the ackA gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome, obtained with primers ackaF and ackaR (Table 3), into the SmaI side of pBAD24
pAS102 pBAD24 bearing the pgi gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers pgiF and pgiR (Table 3), into the SmaI side of pBAD24
pAS103 pBAD24 bearing the fbaB gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers fbabF and fbabR (Table 3), into the KpnI side of pBAD24
pAS104 pBAD24 bearing the tktB gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers tktbF and tktbR (Table 3), into the KpnI side of pBAD24
pAS105 pBAD24 bearing the pta gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers ptaF and ptaR (Table 3), into the KpnI side of pBAD24
pAS106 pBAD24 bearing the gpm gene under of pBAD control This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers gpmaF and gpmaR (Table 3), into the KpnI side of pBAD24
pAS107 pBAD24 bearing the aceB gene under of pBAD control This study by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers acebF and acebR (Table 3), into the KpnI side of pBAD24