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Table 2 Plasmids employed and constructed in this study

From: Genetic response to metabolic fluctuations: correlation between central carbon metabolism and DNA replication in Escherichia coli

Plasmid

Relevant characteristics

Reference

pBAD24

Ori pBR322; bla+ PBAD

[29]

pAS101

pBAD24 bearing the ackA gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome, obtained with primers ackaF and ackaR (Table 3), into the SmaI side of pBAD24

pAS102

pBAD24 bearing the pgi gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers pgiF and pgiR (Table 3), into the SmaI side of pBAD24

pAS103

pBAD24 bearing the fbaB gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers fbabF and fbabR (Table 3), into the KpnI side of pBAD24

pAS104

pBAD24 bearing the tktB gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers tktbF and tktbR (Table 3), into the KpnI side of pBAD24

pAS105

pBAD24 bearing the pta gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers ptaF and ptaR (Table 3), into the KpnI side of pBAD24

pAS106

pBAD24 bearing the gpm gene under of pBAD control

This study, by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers gpmaF and gpmaR (Table 3), into the KpnI side of pBAD24

pAS107

pBAD24 bearing the aceB gene under of pBAD control

This study by cloning of a PCR amplified fragment of E. coli MG1655 chromosome fragment obtained with primers acebF and acebR (Table 3), into the KpnI side of pBAD24

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Microbial Cell Factories

ISSN: 1475-2859

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