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Table 1 E. coli strains used in this work

From: Genetic response to metabolic fluctuations: correlation between central carbon metabolism and DNA replication in Escherichia coli

Strain Relevant characteristics Reference or source
JJC809 (PC8) dnaB8(ts) CmR F2 leuB6 thyA47 deoC3 rps153 l2 [21]
PC2 dnaC( ts) thy leu rpsL [21]
PC3 dnaG(ts) Hfr leu thy rpsL [22]
MG1655 F- λ- ilvG- rfb-50 rph-1 [23]
MG1655dnaA 46 F- λ- ilvG- rfb-50 rph-1 dnaA 46 tna::Tn10 [24]
DH5α F- φ80lacZΔ M15 Δ(lac ZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyr A96 rel A1 λ- [25]
BW25113 Δ(araD-araB)567, ΔlacZ4787::rrnB-3, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 [26]
JW1122 Same as BW25113 but Δicd::kan [27]
JW1413 Same as BW25113 but ΔgapC::kan [27]
JW1666 Same as BW25113 but ΔpykF::kan [27]
JW1841 Same as BW25113 but Δzwf::kan [27]
JW2449 Same as BW25113 but ΔtktB::kan [27]
JW3366 Same as BW25113 but Δpck::kan [27]
JW3890 Same as BW25113 but ΔtpiA::kan [27]
JW3974 Same as BW25113 but ΔaceB::kan [27]
JW3985 Same as BW25113 but Δpgi::kan [27]
JW2294 Same as BW25113 but Δpta::kan [27]
JW2293 Same as BW25113 but ΔackA::kan [27]
JW5173 Same as BW25113 but ΔicdC::kan [27]
JW5344 Same as BW25113 but ΔfbaB::kan [27]
JW0738 Same as BW25113 but ΔgpmA::kan [27]
NR13339 Same as KA796 with dnaN159( Ts) zid501:: Tn10 [28]
NR7651 Same as MC4100 lacZ 104 dnaE486( Ts) zae502:: Tn10 [28]
AS701 MG1655 dnaA46 Δacn::kan This study, by P1 transduction from JW0114
AS702 MG1655 dnaA46 Δicd::kan This study, by P1 transduction from JW1122
AS703 MG1655 dnaA46 ΔgapC::kan This study, by P1 transduction from JW1413
AS704 MG1655 dnaA46 ΔpykF::kan This study, by P1 transduction from JW1666
AS705 MG1655 dnaA46 Δzwf::Kan This study, by P1 transduction from JW1841
AS706 MG1655 dnaA46ΔtktB::kan This study, by P1 transduction from JW2449
AS707 MG1655 dnaA46 Δpck::Kan This study, by P1 transduction from JW3366
AS708 MG1655 dnaA46 ΔtpiA::Kan This study, by P1 transduction from JW3890
AS709 MG1655 dnaA46 ΔaceB:Kan This study, by P1 transduction from JW3974
AS710 MG1655 dna A46 Δpgi::kan This study, by P1 transduction from JW3985
AS711 MG1655 dna A46 Δpta::kan This study, by P1 transduction from JW2294
AS712 MG1655 dnaA46 ΔackA::kan This study, by P1 transduction from JW2293
AS713 MG1655 dnaA46 ΔicdC::kan This study, by P1 transduction from JW5173
AS714 MG1655 dnaA46ΔfbaB::kan This study, by P1 transduction from JW5344
AS715 MG1655 dnaA46 ΔgpmA::kan This study, by P1 transdukcion from JW0738
AS766 MG1655 dnaB8 Δacn::kan This study, by P1 transduction from JW0114
AS767 MG1655 dnaB8 Δicd::kan This study, by P1 transduction from JW1122
AS768 MG1655 dnaB8 ΔgapC::kan This study, by P1 transduction from JW1413
AS769 MG1655 dnaB8 ΔpykF::kan This study, by P1 transduction from JW1666
AS770 MG1655 dnaB8 Δzwf::kan This study, by P1 transduction from JW1841
AS771 MG1655 dnaB8 ΔtktB::kan This study, by P1 transduction from JW1841
AS772 MG1655 dnaB8 Δpck::kan This study, by P1 transduction from JW3366
AS773 MG1655 dnaB8 ΔtpiA::kan This study, by P1 transduction from JW3890
AS774 MG1655 dnaB8 ΔaceB::kan This study, by P1 transduction from JW3974
AS775 MG1655 dnaB8 Δpgi::kan This study, by P1 transduction from JW3985
AS776 MG1655 dnaB8 Δpta::kan This study, by P1 transduction from JW2294
AS778 MG1655 dnaB8 ΔackA::kan This study, by P1 transduction from JW2293
AS779 MG1655 dnaB8 ΔicdC::kan This study, by P1 transduction from JW5173
AS780 MG1655 dnaB8 ΔfbaB::kan This study, by P1 transduction from JW5344
AS781 MG1655 dnaB8 ΔgpmA::kan This study, by P1 transduction from JW0738
AS750 PC2 dnaC(ts) Δacn::kan This study, by P1 transduction from JW0114
AS751 PC2 dnaC(ts) Δicd::kan This study, by P1 transduction from JW1122
AS752 PC2 dnaC(ts) ΔgapC::kan This study, by P1 transduction from JW1413
AS753 PC2 dnaC(ts) ΔpykF::kan This study, by P1 transduction from JW1666
AS754 PC2 dnaC(ts) Δzwf::kan This study, by P1 transduction from JW1841
AS755 PC2 dnaC(ts) ΔtktB::kan This study, by P1 transduction from JW2449
AS756 PC2 dnaC(ts) Δpck::kan This study, by P1 transduction from JW3366
AS757 PC2 dnaC(ts) ΔtpiA::kan This study, by P1 transduction from JW3890
AS758 PC2 dnaC(ts) ΔaceB::kan This study, by P1 transduction from JW3974
AS759 PC2 dnaC(ts) Δpgi::kan This study, by P1 transduction from JW3985
AS760 PC2 dnaC(ts) Δpta::kan This study, by P1 transduction from JW2294
AS761 PC2 dnaC(ts) ΔackA::kan This study, by P1 transduction from JW2293
AS762 PC2 dnaC(ts) ΔicdC::kan This study, by P1 transduction from JW5173
AS763 PC2 dnaC(ts) ΔfbaB::kan This study, by P1 transduction from JW5344
AS764 PC2 dnaC(ts) ΔgpmA::kan This study, by P1 transduction from JW0738
AS783 PC3 dnaG(ts) Δacn::kan This study, by P1 transduction from JW0114
AS784 PC3 dnaG(ts) Δicd::kan This study, by P1 transduction from JW1122
AS785 PC3 dnaG(ts) ΔgapC::kan This study, by P1 transduction from JW1413
AS786 PC3 dnaG(ts) ΔpykF::kan This study, by P1 transduction from JW1666
AS787 PC3 dnaG(ts) Δzwf::kan This study, by P1 transduction from JW1841
AS788 PC3 dnaG(ts) ΔtktB::kan This study, by P1 transduction from JW2449
AS789 PC3 dnaG(ts) Δpck::kan This study, by P1 transduction from JW3366
AS790 PC3 dnaG(ts) ΔtpiA::kan This study, by P1 transduction from JW3890
AS791 PC3 dnaG(ts) ΔaceB::kan This study, by P1 transduction from JW3974
AS792 PC3 dnaG(ts) Δpgi::kan This study, by P1 transduction from JW3985
AS793 PC3 dnaG(ts) Δpta::kan This study, by P1 transduction from JW2294
AS794 PC3 dnaG(ts) ΔackA::kan This study, by P1 transduction from JW2293
AS795 PC3 dnaG(ts) ΔicdC::kan This study, by P1 transduction from JW7173
AS796 PC3 dnaG(ts) ΔfbaB::kan This study, by P1 transduction from JW5344
AS797 PC3 dnaG(ts) ΔgpmA::kan This study, by P1 transduction from JW0738
AS718 MG1655 dnaE486 Δacn This study, by P1 transduction from JW0114
AS719 MG1655 dnaE486 Δicd This study, by P1 transduction from JW1122
AS720 MG1655 dnaE486 ΔgapC This study, by P1 transduction from JW1413
AS721 MG1655 dnaE486 ΔpykF This study, by P1 transduction from JW1666
AS722 MG1655 dnaE486 Δzwf This study, by P1 transduction from JW1841
AS723 MG1655 dnaE486 ΔtktB This study, by P1 transduction from JW2449
AS724 MG1655 dnaE486 Δpck This study, by P1 transduction from JW3366
AS725 MG1655 dnaE486 ΔtpiA This study, by P1 transduction from JW3890
AS726 MG1655 dnaE486 ΔaceB This study, by P1 transduction from JW3974
AS728 MG1655 dnaE486 Δpgi This study, by P1 transduction from JW3985
AS729 MG1655 dnaE486 Δpta This study, by P1 transduction from JW2294
AS730 MG1655 dnaE486 ΔackA This study, by P1 transduction from JW2293
AS731 MG1655 dnaE486 ΔicdC This study, by P1 transduction from JW5173
AS732 MG1655 dnaE486 ΔfbaB This study, by P1 transduction from JW5344
AS733 MG1655 dnaE486 ΔgpmA This study, by P1 transduction from JW0738
AS734 MG1655 dnaN159 ΔacnB::kan This study, by P1 transduction from JW0114
AS735 MG1655 dnaN159 Δicd::kan This study, by P1 transduction from JW1122
AS736 MG1655 dnaN159 ΔgapC::kan This study, by P1 transduction from JW1413
AS737 MG1655 dnaN159 ΔpykF::kan This study, by P1 transduction from JW1666
AS738 MG1655 dnaN159 Δzwf::kan This study, by P1 transduction from JW1841
AS739 MG1655 dnaN159 ΔtktB::kan This study, by P1 transduction from JW2449
AS740 MG1655 dnaN159 Δpck::kan This study, by P1 transduction from JW3366
AS741 MG1655 dnaN159 ΔtpiA::kan This study, by P1 transduction from JW3890
AS742 MG1655 dnaN159 ΔaceB::kan This study, by P1 transduction from JW3974
AS743 MG1655 dnaN159 Δpgi::kan This study, by P1 transduction from JW3985
AS744 MG1655 dnaN159 Δpta::kan This study, by P1 transduction from JW2294
AS745 MG1655 dnaN159 ΔackA::kan This study, by P1 transduction from JW2293
AS746 MG1655 dnaN159 ΔicdC::kan This study, by P1 transduction from JW5173
AS747 MG1655 dnaN159 ΔfbaB::kan This study, by P1 transduction from JW5344
AS748 MG1655 dnaN159 ΔgpmA::kan This study, by P1 transduction from JW0738
AS700 MG1655 dnaN159 zid501:: Tn10 This study, by P1 transduction from NR13339
AS717 MG1655 dnaE486 zae502:: Tn10 This study, by P1 transduction from NR7651
AS765 MG1655 dnaB8(ts) cmR This study, by P1 transduction from JJC809