Figure 5From: Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yieldP2000 and SB2121 do not affect DO in shake flasks. Addition of 1% P2000 (A) or 1% SB2121 (B) did not affect the DO in shake flasks, as determined by DO fluorescent sensors (PreSens) attached to the underside of each flask. The experiments in panels A and B are biologically independent, which is the origin of the clonal differences and the variations in technical additions.Back to article page