Figure 1
From: Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield
Antifoam addition increases the total yield of GFP in 20 mL P. pastoris cultures. Bar charts showing the total yield of GFP (μg) at 48 h in 20 mL P. pastoris cultures following addition of Antifoam A (A), Antifoam C (B), J673A (C), P2000 (D) and SB2121 (E) at concentrations from 0-1%. The error bars show the respective standard deviations. In all cases n = 9. The numbers within each bar are the corresponding specific yield (μg OD595-1) with the respective standard deviations in parentheses (n = 9). The horizontal line is a visual aid to link the mean optical density (grey squares) for each concentration of antifoam across the full experimental range; error bars show the respective standard deviations (n = 9). In each case a one-way ANOVA showed that P < 0.001. Asterisks show the significance of the total yield and specific yield data for each antifoam concentration compared to the respective 0% antifoam control as determined by a Dunnett's multiple comparison test, where * = P ≤ 0.05 and ** = P ≤ 0.01.