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Table 4 Analysis of plasmid production and cell growth in different commercially available media and PDM-pcDNA3F.

From: Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques

Medium

pcDNA3F volumetric yield*

pcDNA3F specific yield*

pcDNA3F production rate*

Final cell density*

Cell growth rate*

 

(mg/L)

(mg/g)

(mg/L/h)

(g/L)

(h-1)

Terrific Broth

8.40

4.31

0.56

1.95

0.85

Luria Broth

5.80

7.94

0.39

0.73

0.31

2xYT

6.42

3.82

0.43

1.68

0.77

PDM

7.80

3.57

0.52

2.18

0.89

PDM-pcDNA3F

13.65

9.10

0.91

1.50

0.75

  1. *Cells were cultured in 250 ml of medium containing 100 μg/mL ampicillin using 500 mL shake flasks at 200 rpm, 37°C and halted after 15 h. Each value represents an average of triplicates. Standard deviations for all data are 1 - 5% of the values shown.