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Table 1 Composition of basic (PDM) and optimised (PDM-pcDNA3F) medium for DH5α-pcDNA3F cultivation.

From: Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques

Nutrient

PDM

(g/L)

PDM-pcDNA3F

(g/L)

yeast extract

4.41

4.41

tryptone

7.93

10.60

glucose

10.00

6.50

Na2HPO4

12.80

15.70

K2HPO4

3.00

3.00

NH4Cl

0.50

0.50

MgSO4

0.24

0.24