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Figure 7 | Microbial Cell Factories

Figure 7

From: Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques

Figure 7

Ethidium bromide agarose gel electrophoresis of pDNA samples from fed batch fermentation of DH5α-pcDNA3F. Loading volume = 1 μL. Analysis was performed using 1% agarose in TAE × 1 buffer, 3 μg/mL ethidium bromide at 65 V for 90 min. Lane M is 1 kb DNA ladder and lane P represents 55.0 mg/L of purified pcDNA3F.

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Microbial Cell Factories

ISSN: 1475-2859

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