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Figure 2 | Microbial Cell Factories

Figure 2

From: Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques

Figure 2

Effect of different growth temperature induction schemes on plasmid production. Temperature inductions were performed at 8 h post inoculation. Cells were cultured in 250 ml PDM medium using 500 mL shake flasks at 200 rpm, initial temperature of 37°C and halted after 15 h. Each data point represents an average of five replicates (n = 5). (A) Profiles of an actual (dashed line) and a model (solid line) growth temperature fluctuations. (B) Effect of frequency of temperature fluctuation (1/f) on plasmid specific yield. (C) Analysis of plasmid volumetric and specific yields from different temperature induction schemes.

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Microbial Cell Factories

ISSN: 1475-2859

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