Figure 2

Refolding efficiency of prochymosin prepared by expressing codon-optimized genes in E. coli W3110. Renaturation was carried out by diluting the urea-solubilized inclusion bodies in renaturation buffer at a final protein concentration of 1 mg/ml and incubating the mixture at 4°C for 12 h. Other experimental details are provided in the methods section. Values shown are means of three independent determinations. The standard deviations were in all the cases less than 10% of the corresponding means.