A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Table 2 Comparison of the specific substrate uptake rates q_s and determined maximum specific productivities q_p.

Experiment^a	Methanol concentration	Specific substrate uptake rate q_s[mmol·g^-1·h^-1]	Specific productivity q_p[U·g^-1·h^-1]
Batch with methanol pulses	from 0 to 16 g/L	~ 2	2.5 ± 0.25
Fed batch A ▲	limited	< 1	2.0 ± 0.20
Fed batch B ◆	limited	1.75	5.0 ± 0.50
Fed batch C1 ▼	limited	stepwise up to 2	10 ± 0.10
Fed batch C2 ●	limited	stepwise up to 2	11 ± 0.11

^a all experiments were performed in the presence of different concentrations of the inducer methanol

ISSN: 1475-2859