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Table 2 Comparison of the specific substrate uptake rates qs and determined maximum specific productivities qp.

From: A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Experimenta Methanol concentration Specific substrate uptake rate qs[mmol·g-1·h-1] Specific productivity qp[U·g-1·h-1]
Batch with methanol pulses from 0 to 16 g/L ~ 2 2.5 ± 0.25
Fed batch A ▲ limited < 1 2.0 ± 0.20
Fed batch B limited 1.75 5.0 ± 0.50
Fed batch C1 limited stepwise up to 2 10 ± 0.10
Fed batch C2 limited stepwise up to 2 11 ± 0.11
  1. a all experiments were performed in the presence of different concentrations of the inducer methanol