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Figure 1 | Microbial Cell Factories

Figure 1

From: A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Figure 1

Experimental strategy for the determination of relevant strain specific parameters in a batch cultivation with methanol pulses. These strain specific data (Δtimeadapt - adaptation time; qs adapt - specific substrate uptake rate during the adaptation period; qs max - maximum specific substrate uptake rate) are then used to set up a feeding profile for P. pastoris in fed batch cultivations. The calculated yields (YX/Y, YCO2/S) and the specific productivity (qp) can consequently be used for strain characterization.

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