Figure 1

Experimental strategy for the determination of relevant strain specific parameters in a batch cultivation with methanol pulses. These strain specific data (Δtime_adapt - adaptation time; q_{s adapt} - specific substrate uptake rate during the adaptation period; q_{s max} - maximum specific substrate uptake rate) are then used to set up a feeding profile for P. pastoris in fed batch cultivations. The calculated yields (Y_X/Y, Y_CO2/S) and the specific productivity (q_p) can consequently be used for strain characterization.