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Table 3 ANOVA for Response Surface Quadratic Model

From: Comparative one-factor-at-a-time, response surface (statistical) and bench-scale bioreactor level optimization of thermoalkaline protease production from a psychrotrophic Pseudomonas putida SKG-1 isolate

Source

Sum of squares

df

Mean square

F-value

p-value

Model

1.205E+005

14

8609.99

820.46

< 0.0001

A-Glucose

3675.00

1

3675.00

350.20

< 0.0001

B-Yeast extract

2790.75

1

2790.75

265.94

< 0.0001

C-MgSO4

1140.75

1

1140.75

108.70

< 0.0001

D-pH

69008.33

1

69008.33

6575.95

< 0.0001

AB

6.25

1

6.25

0.60

0.4531

AC

0.25

1

0.25

0.024

0.8795

AD

256.00

1

256.00

24.39

0.0002

BC

324.00

1

324.00

30.87

< 0.0001

BD

1024.00

1

1024.00

97.58

< 0.0001

CD

16.00

1

16.00

1.52

0.2372

A2

274.05

1

274.05

26.12

0.0002

B2

30325.24

1

30325.24

2889.76

< 0.0001

C2

13950.10

1

13950.10

1329.33

< 0.0001

D2

555.00

1

555.00

52.89

< 0.0001

Residual

146.92

14

10.49

  

Lack of fit

146.92

10

14.69

  

Pure error

0.000

4

0.000

  

Cor total

1.205E+005

28

   

Standard deviation

3.24

R-squared

0.9988

Mean

465.38

Adjusted R-squared

0.9976

Coefficient of variation (C.V.%)

0.70

Predicted R-squared

0.9930

PRESS

846.24

Adequate precision

121.595