Table 2 Experimental designs used in RSM studies by using four independent variables with six centre points showing observed and predicted values of protease production
Standard Order | Factor A (Glucose %, w/v) | Factor B (Yeast extract %, w/v) | Factor C (MgSO4 %, w/v ) | Factor D (pH) | Observed response (Uml-1) | Predicted response (Uml-1) |
|---|---|---|---|---|---|---|
1 | 0.75 | 0.30 | 0.0100 | 9.00 | 419 | 418.13 |
2 | 1.25 | 0.30 | 0.0100 | 9.00 | 457 | 455.63 |
3 | 0.75 | 0.70 | 0.0100 | 9.00 | 449 | 451.13 |
4 | 1.25 | 0.70 | 0.0100 | 9.00 | 482 | 483.63 |
5 | 1.00 | 0.50 | 0.0075 | 8.80 | 524 | 562.46 |
6 | 1.00 | 0.50 | 0.0125 | 8.80 | 540 | 541.96 |
7 | 1.00 | 0.50 | 0.0075 | 9.20 | 372 | 370.79 |
8 | 1.00 | 0.50 | 0.0125 | 9.20 | 396 | 394.29 |
9 | 0.75 | 0.50 | 0.0100 | 8.80 | 561 | 561.58 |
10 | 1.25 | 0.50 | 0.0100 | 8.80 | 617 | 612.58 |
11 | 0.75 | 0.50 | 0.0100 | 9.20 | 426 | 425.92 |
12 | 1.25 | 0.50 | 0.0100 | 9.20 | 447 | 444.92 |
13 | 1.00 | 0.30 | 0.0075 | 9.20 | 369 | 365.25 |
14 | 1.00 | 0.70 | 0.0075 | 9.00 | 415 | 413.75 |
15 | 1.00 | 0.30 | 0.0125 | 9.00 | 406 | 402.75 |
16 | 1.00 | 0.70 | 0.0125 | 9.00 | 416 | 415.25 |
17 | 0.75 | 0.50 | 0.0075 | 9.00 | 446 | 446.63 |
18 | 1.25 | 0.50 | 0.0075 | 9.00 | 479 | 482.13 |
19 | 0.75 | 0.50 | 0.0125 | 9.00 | 466 | 466.63 |
20 | 1.00 | 0.50 | 0.0125 | 9.00 | 498 | 501.13 |
21 | 1.00 | 0.30 | 0.0125 | 8.80 | 509 | 512.96 |
22 | 1.00 | 0.70 | 0.0100 | 8.80 | 513 | 511.46 |
23 | 1.00 | 0.30 | 0.0100 | 9.20 | 324 | 329.29 |
24 | 1.00 | 0.70 | 0.0100 | 9.20 | 392 | 391.79 |
25 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
26 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
27 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
28 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
29 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
30 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |