From: Comparative one-factor-at-a-time, response surface (statistical) and bench-scale bioreactor level optimization of thermoalkaline protease production from a psychrotrophic Pseudomonas putida SKG-1 isolate
Variables
Levels
-1
0
+1
Glucose (%, w/v)
0.75
1.00
1.25
Yeast extract (%, w/v)
0.30
0.50
0.70
MgSO4 (%, w/v)
0.0075
0.0100
0.0125
pH
8.80
9.00
9.20