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Table 2 Apparent kinetic constants of purified rCDH for cellobiose

From: Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

Electron acceptor K M (μM) V max a (μmol/min/mg) k cat (s-1) k cat/K M (s-1. mM-1)
DCPIP 35.5 22.2 40.8 1148
Cyt c 14.7 3.9 7.0 474
  1. Reactions were performed at 30°C, pH 4.5 using selected electron acceptors.
  2. a Kinetic parameters were determined using DCPIP or cyt c as electron acceptor and cellobiose as substrate under standard assay conditions.