Analysis of total reducing sugar yield after 96 h of enzymatic treatment on wheat straw. Reducing sugars (mM) release during hydrolysis were quantified spectrophotometrically using the DNS method as described in Navarro et al., 2010 . Assay conditions for hydrolysis were conducted in 20 mL of 50 mM sodium phosphate buffer (pH 4.8) containing 5% (w/v) wheat straw. (A) P. cinnabarinus secretome containing CDH or (B) purified rCDH were assayed in the presence of cellulose cocktail at 45°C with an orbital agitation (140 rpm) during 96 h. C: control reaction without CDH. Error bars are for triplicate, each measured once.