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Figure 3 | Microbial Cell Factories

Figure 3

From: The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide

Figure 3

SDS-PAGE analysis of purified recombinant pro-TGase and TGase. 1: supernatants of E. coli cells harboring pSPTG; 2, 3: active fractions of nickel-affinity and the following gel-filtration chromatography of supernatants of E. coli cells harboring pSPTG, respectively; 4: intracellular soluble fraction of E. coli cells harboring pSPRO-STG; 5, 6: active fractions of nickel-affinity and the following gel-filtration chromatography of the intracellular soluble fraction of E. coli cells harboring pSPRO-STG, respectively.

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