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Figure 2 | Microbial Cell Factories

Figure 2

From: The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide

Figure 2

Expression of pro-TGase and TGase in E. coli. (A) SDS-PAGE analysis of TGase expression using single expression vectors. 1, 2: insoluble fractions of E. coli cells harboring pSTG and pTG, respectively; 3, 4: intracellular soluble fractions of cells harboring pSTG and pTG, respectively. 5, 6: supernatant and whole cell extracts of E. coli harboring pSPTG, respectively. (B) SDS-PAGE analysis of TGase expression using co-expression constructs (intracellular soluble fractions). (C) SDS-PAGE analysis of TGase expression using co-expression vectors (insoluble fractions). (D) Intracellular TGase activity of E. coli cells harboring the co-expression constructs. 1-9: E. coli cells harboring pTG-PRO, pTG-SPRO, pSTG-PRO, pSTG-SPRO, pPRO-TG, pPRO-STG, pSPRO-TG, pSPRO-STG, and pSPROT7STG, respectively. The bands of TGase and pro-TGase are indicated by downward and upward arrows, respectively.

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Microbial Cell Factories

ISSN: 1475-2859

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