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Table 2 Theoretical peptides generated by LysC digestion of rAPA from S. lividans.

From: The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks

Fragment Cutting Position Amino acid sequence Peptide length Mass (Da)
P1 106 DPEPAPPVPTTAASPPSTAA APPAPATPVAPPPPAAANTP NAQPGDPNAAPPPADPNAP PPVIAPNAPQPVRIDNPVGG FSFALPAGWVESDAAHFDYG SALLSK 106 10310.439
P2 134 TTGDPPFPGQPPPVANDTRI VLGRLDQK 28 2987.365
P3 145 LYASAEATDSK 11 1155.227
P4 188 AAARLGSDMGEFYMPYPGTR INQETVSLDANGVSGSASYY EVK 43 4604.056
P5 194 FSDPSK 6 679.728
P6 234 PNGQIWTGVIGSPAANAPDA GPPQRWFVVWLGTANNPVDK 40 4199.698
P7 238 GAAK 4 345.399
P8 286 ALAESIRPLVAPPPAPAPAP AEPAPAPAPAGEVAPTPTTP TPQRTLPA 48 4624.317