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Table 1 Morphological differences in cultures carried out in conventional Erlenmeyer normal (NF), baffled (BF), and coiled shake flasks (CF). Data are presented as average ± standard deviation of image analysis of at least 100 clumps, mycelia or pellets for each sample, and at least 3 samples were analyzed for each flask. Values with the same superscript (a,b or c) are not significantly different by Tukey HSD Test (p = 0.01).

From: The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks

Shake flask

Area (mm2)

Diameter (mm)

Perimeter (mm)

Roundness (-)

Conventional

2.11 ± 1.22

1.57 ± 0.41

6.15 ± 2.32

1.51 ± 0.49

Baffled

0.04 ± 0.02 a

0.23 ± 0.06 b

1.01 ± 0.35 c

2.11 ± 0.67

Coiled

0.02 ± 0.01 a

0.16 ± 0.05 b

0.77 ± 0.30 c

2.66 ± 0.98

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Microbial Cell Factories

ISSN: 1475-2859

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