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Table 1 Properties of the RK2-based broad-host range plasmid constructs with the Ptac/lacIq (pSEVAlac) or Pm/xylS (pSEVAxyl) promoter/regulator gene systems used in this study.

From: Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

Construct

scFv specificity

Promoter

Affinity-tags

codon optimization1

% GC2

pSEVAlacD1.3n

hen egg-white lysozyme

Ptac

HIS6/Myc/Strep-tag® II

_

52,3

pSEVAlacTOB5-D4n

human CRP

Ptac

Myc/HIS6/Strep-tag® II

_

55,0

pSEVAlacTOB5-D4s

human CRP

Ptac

Myc/HIS6/Strep-tag® II

Jcat/Eurofins MWG Operon

68,3

pSEVAxylTOB5-D4s

human CRP

Pm

Myc/HIS6/Strep-tag® II

Jcat/Eurofins MWG Operon

68,3

pSEVAlacHT186-D11n

human mucin1

Ptac

HIS6/Myc/Strep-tag® II

_

57,8

pSEVAlacHT186-D11s

human mucin1

Ptac

HIS6/Myc/Strep-tag® II

GeneOptimizer®/GENEART

67,6

pSEVAxylHT186-D11s

human mucin1

Pm

HIS6/Myc/Strep-tag® II

GeneOptimizer®/GENEART

67,6

  1. 1Synthetic scFv determinants with optimized codon usage: - indicates the original scFv sequence, Jcat/Eurofins MWG Operon and GeneOptimizer®/GENEART indicates a codon adaptation tool and a supplier; 2 %GC content of the scFv coding sequence.