Skip to main content

Advertisement

Table 1 Properties of the RK2-based broad-host range plasmid constructs with the Ptac/lacIq (pSEVAlac) or Pm/xylS (pSEVAxyl) promoter/regulator gene systems used in this study.

From: Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

Construct scFv specificity Promoter Affinity-tags codon optimization1 % GC2
pSEVAlacD1.3n hen egg-white lysozyme Ptac HIS6/Myc/Strep-tag® II _ 52,3
pSEVAlacTOB5-D4n human CRP Ptac Myc/HIS6/Strep-tag® II _ 55,0
pSEVAlacTOB5-D4s human CRP Ptac Myc/HIS6/Strep-tag® II Jcat/Eurofins MWG Operon 68,3
pSEVAxylTOB5-D4s human CRP Pm Myc/HIS6/Strep-tag® II Jcat/Eurofins MWG Operon 68,3
pSEVAlacHT186-D11n human mucin1 Ptac HIS6/Myc/Strep-tag® II _ 57,8
pSEVAlacHT186-D11s human mucin1 Ptac HIS6/Myc/Strep-tag® II GeneOptimizer®/GENEART 67,6
pSEVAxylHT186-D11s human mucin1 Pm HIS6/Myc/Strep-tag® II GeneOptimizer®/GENEART 67,6
  1. 1Synthetic scFv determinants with optimized codon usage: - indicates the original scFv sequence, Jcat/Eurofins MWG Operon and GeneOptimizer®/GENEART indicates a codon adaptation tool and a supplier; 2 %GC content of the scFv coding sequence.