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Figure 1 | Microbial Cell Factories

Figure 1

From: Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

Figure 1

Purity of single-step affinity-purified scFvs from KT2440. Proteins were isolated, affinity purified, incubated and subjected to freeze:thaw cycles to assess their stability in elution buffer, as described above and run on a 12.5% SDS polyacrylamide gel. M = PAGE Ruler unstained protein molecular weight marker (Fermentas); 1, D1.3; 2, TOB5-D4 (anti-CRP), 3, HT186-D11 (anti-MUC1).

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