Figure 3

Analysis of the expression of anti-VLY VP1/VP2-scFv-Fv pseudotype VLPs in yeast. (A): Coomassie blue-stained SDS-PAGE; (B): Western blot with HRP-conjugated anti- human IgG; (C): Western blot with mouse polyclonal antibodies against VP1/VP2 VLPs. The same samples were run on each gel. In lanes: 1-negative control sample from whole crude lysate of S. cerevisiae cells, transformed with empty vector pFX7; 2-whole crude lysate of yeast transformed with pFGG3-VP1/VP2-scFv-Fc plasmid, 3-the soluble fraction recovered after centrifugation of crude lysate of yeast transformed with pFGG3-VP1/VP2-scFv-Fc plasmid, 4-VLPs consisting of VP1 protein and fusion protein VP2-scFv-Fc purified using sucrose and CsCl gradients; M-pre-stained protein weight marker (Thermo Scientific Fermentas). (D): Electron microscopy pictures of VP1/VP2-scFv-Fv pseudotype VLPs, stained with 2% aqueous uranyl acetate solution and examined by Morgagni 268 electron microscope.