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Figure 5 | Microbial Cell Factories

Figure 5

From: High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Figure 5

Adh activity per biomass. Specific activity (units per mg of cell dry weight) of the recombinant Adh product expressed in E. coli RB791 in EnBase, TB and ZYM cultures in Ultra Yield and Erlenmeyer flasks. 1 unit is defined as the amount of ADH required for conversion of 1 mM substrate min-1 at 20°C and pH 7.0. EB: EnBase; TB: Terrific Broth; ZYM: ZYM-5052 autoinduction medium; UY: Ultra Yield Flask; Erl: Erlenmeyer flask. The values 0.6, 1.5, 3 and 6 associated with EnBase cultures on the x axis refer to glucose-releasing enzyme concentrations in U l-1.

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Microbial Cell Factories

ISSN: 1475-2859

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